hTROP2/CT26
CBPG0026
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| I. Background | |
| TROP2 由 TACSTD2 基因编码，是一种钙信号跨膜蛋白，TROP2 在胚胎发育过程中以 及恶性肿瘤细胞的增殖、侵袭、转移具有重要意义。TROP2 在很多健康的器官中表达，包 括呼吸道、宫颈、子宫内膜、结肠直肠和胆管上皮的肝脏等发育正常成熟的器官外，胚胎 和胎儿发育过程中 TROP2 也会在肺、肠、胃、膀胱和肾脏中表达。TROP2 在各种上皮性 肿瘤中特异表达，顺利获得增加细胞中周期蛋白 D1 和 E 的 量来加速细胞周期过程，从而导致 肿瘤的生长，明显提高肿瘤细胞的侵袭力和转移潜能。TROP2 在胰腺癌、乳腺癌、结肠癌、 胃癌、口腔鳞癌等多种上皮起源的实体肿瘤中高表达，在男性前列腺癌中和女性卵巢癌、 宫颈癌等生殖系统恶性肿瘤也高表达。TROP2 的高表达也存在于非上皮起源的肿瘤中，例 如黑色素瘤、结外 NK/T 细胞淋巴瘤、胶质瘤和胶质母细胞瘤以及骨肉瘤。TROP2 在垂体 腺瘤中也被发现过表达。 | |
| II. Introduction | |
| Host Cell: | CT26 |
| Expressed gene: | TROP2 |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640+10%FBS+2 μg/ml Puromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| III. Description of Host Cell Line | |
| Organism: | Mouse |
| Tissue: | colon, metastatic |
| Morphology: | N/A |
| Growth Properties: | monolayer |
| IV. Representative Data | |
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Figure 1. Recombinant hTROP2/CT26 stably expressing TROP2. |
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| V. In VIVO Data | |
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Index |
Content |
|
Animal |
BALB/C, 6weeks, female |
|
Implanted Position |
Subcutaneous (right lower flank region ) |
|
Injected Cell Numbers |
5×10^5/0.1ml |
|
Matrix gel |
Without matrix gel |
1. Weight Change
Figure 2. hTROP2/CT26 Mean Body Weight ± SEM.
2. Tumor Size
Figure 3.hTROP2/CT26 Mean Tumor Volume ± SEM .
NOTE：The above data was provided by Cobioer (cell data) and Truway Bio (in vivo data model).
