BT474-Luc
CBP30103L
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| I. Introduction | |
| Host Cell: | BT-474 HER2 amplification |
| Expressed gene: | Luciferase |
| Stability: | 32 passages(in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM+10%FBS+0.2%BT474 advance+2ug/ml puromycin |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen |
| II. Description of Host Cell Line | |
| Organism: | Homo sapiens,human |
| Tissue: | Mammarygland;breastduct |
| Disease: | Ductal carcinoma |
| Morphology: | Epithelial |
| Growth Properties: | Adherent,patchy The cells form adherent patches of epithelial-like cells The patches are compact multilayered colonies that rarely become confluent |
| III. Representative Data | |
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Figure. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer). The number of Luminescence signal is 484±16(Mean±SD) on BT474 cells while 691026±25124(Mean±SD) on BT474-Luc cells. |
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| IV. Stablity Test | |
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Figure. Stability test results. BT474-Luc cells showed at least 32 passage stability with similar luminescence unit. |
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